BD CaliBRITE beads are designed for use with FACSComp or AutoCOMP software and the FACS family of flow cytometers (FACSCalibur, FACSort, FACScan. values for BD Calibrite beads. To edit, see page A Target file is also created for. HLA-B Although used by. BD FACSComp software, the file is not editable. Product Name: BD CALIBRITE BEADS. Synonyms: BD CALIBRITE BEADS; CALIBRITE BEADS. CAS: MF: MW: 0. EINECS: Mol File: Mol File. BD CALIBRITE .
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NOTE Over a period of time, the fluorescence separation might decrease. In some cases the software may not be able to automatically set up the instrument. The drop should be cloudy, indicating the beads are properly mixed.
Corrective action might be required if the average separation varies by more than 2. Notice populations with a lower FSC signal than lymphocytes debris, for example can be excluded by increasing the FSC threshold level. This product is licensed for sale only for in vitro diagnostics. If deterioration is suspected, prepare a calkbrite bead suspension and check instrument conditions. Label two 12 x mm polystyrene tubes Tube A and Tube B.
BD Calibrite™ Beads
Following PMT and compensation adjustment, the software performs a Sensitivity Test using the appropriate mixed-bead suspension. The flow cytometer has separate detectors or photomultiplier tubes PMTs that detect light signals. Generate a printout of the Sensitivity Test results and keep the printouts in a log calibrihe. The czlibrite in separation for a wide variety of bead lots has been within 2. See examples in Optimization and Quality Control on page 4. Compensation adjustments for FL1, FL2, and FL3 correct for spectral overlap by shifting the labeled bead calibrife so they are aligned with the corresponding unlabeled bead populations.
This instructions for use IFU provides information for two- three- and four-color setup. Observations of greater variations on a single instrument can be indicative of instrument instability.
One bottle is sufficient to perform 25 tests. Mix bead vials by gentle inversion or very gentle vortexing prior to use.
BD Calibrite™ – BD Calibrite PerCP-Cy Beads – BD Biosciences
Always refer to the appropriate application note or reagent IFU. Beads used beyond their stability begin to show a decrease in separation between unlabeled and labeled populations, resulting in Sensitivity Test failure.
It might be necessary to adjust the FSC and SSC amplifiers so that all leucocyte populations are on scale, and to adjust compensation and threshold settings see Figure 1. An APC-labeled bead is available separately and may be used with the 3-color kit to beafs four-color setup. See Optimization and Quality Control on page 4.
A thorough investigation of sucrose Figure 1 through Figure 4 show examples of optimization for two- three- and four-color applications. Daily use is recommended for monitoring instrument performance over time.
NOTE Different immunophenotyping preparation methods might require different optimization procedures. The 3-color kit contains these beads plus a PerCPlabeled bead.
BD Calibrite™ Beads
Forward scatter FSC and side scatter SSC instrument sensitivity are measured by the mean channel separation between the light-scatter signal of the beads and background signal electronic and optical. Record PMT voltages and channel separations obtained for each parameter in a daily log sheet. Wellington, Auckland, New Zealand bdbiosciences. Make sure to obtain a full drop of beads.
A minimum channel separation must be met for the scatter and fluorescence parameters. How to make the beaded number line. If this occurs, manually adjust the settings. For further assistance, contact your BD Biosciences service representative. Preparation of Test Suspensions Prepare all bead suspensions immediately prior to use. Perform a Sensitivity Test using Tube B.
The FSC threshold is adjusted to a level that minimizes background signal if any.